chlorogenic acid chl  (LGC Standards)

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Effects of almond skin extract (AE) and CHL on SIR2 ( a ), SOD1 ( b ) and SOD2 ( c ) gene expression determined by RT-qPCR. Expression was normalized with TUB1 gene and expressed relative to young yeast cells (Y). Y CTL was young yeast (on day 2 of cultivation) and A CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, Control, Extraction

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Effects of AE and CHL on SIRT1/SIR2 ( a ) and total SOD ( b ) enzyme activities. Y-CTL was young yeast (on day 2 of cultivation) and A-CTL was aged yeast (on day 5 of cultivation). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. In young yeast culture (Y), actual SIRT1/SIR2 enzyme activity was equal to 4564.4 FU/mg protein, whereas total SOD activity was 35.6 units/mg protein. The enzyme activity was expressed as a percentage of Y-CTL. Values are means ± standard deviations (SD) of 4 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Control, Activity Assay, Extraction

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Survival and ROS/RNS production under UV-C-induced oxidative stress conditions. ( a ) Survival assay, plates were incubated at 28 °C for 3 d and the number of colonies was counted; excepted if mentioned CTL was DBY746 yeast subjected to UV-C stress. ( b ) ROS/RNS production was evaluated using the dihydrorhodamine 123 (DHR123) probe. AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 6 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Clonogenic Cell Survival Assay, Incubation, Control, Extraction

Journal: Life

Article Title: Almond Skin Extracts and Chlorogenic Acid Delay Chronological Aging and Enhanced Oxidative Stress Response in Yeast

doi: 10.3390/life10060080

Figure Lengend Snippet: Schematic view of the different ROS/RNS-induced oxidative damages to membrane lipids, proteins and DNA ( a ), and effects of AE and CHL on malondialdehyde (MDA) levels measured by TBARS assay ( b ), protein carbonyl contents determined by ELISA assay ( c ) and 8-oxo-Guanine formation determined by ELISA assay ( d ). AE, almond extract (1 mg/mL). CHL: chlorogenic acid at 3 concentrations (CHL5, CHL10 and CHL25 corresponding to chlorogenic acid addition at 5, 10 and 25 µM, respectively). E-Resveratrol (RES, 10 µM) was used as control antiaging drug. Values are means ± standard deviations (SD) of 6 independent experiments. Different letters represent significant differences between the various extraction conditions ( p < 0.05).

Article Snippet: Chlorogenic acid (CHL) and resveratrol (RES) standard was purchased from LGC Standard (Molsheim, France).

Techniques: Membrane, TBARS Assay, Enzyme-linked Immunosorbent Assay, Control, Extraction

Journal: Plants

Article Title: The Influence of Phytosociological Cultivation and Fertilization on Polyphenolic Content of Menthae and Melissae folium and Evaluation of Antioxidant Properties through In Vitro and In Silico Methods

doi: 10.3390/plants11182398

Figure Lengend Snippet: ( a ) Menthae extract, entire spectra, ESI−; (A)—MM E and (B)—MF E; ( b ) Melissae extract, entire spectra, ESI−; (A)—MLM E and (B)—MLF E. Legend: PRO—Protocatechuic acid, RUT—Rutin, CAF—Caffeic acid, CHL—Chlorogenic acid, LUT—Luteolin, KAE—Kaempferol, ROS—Rosmarinic acid, QUE—Quercetin, ISO—Isoquercitrin, FER—Ferulic acid, COU— p -Coumaric acid.

Article Snippet: Reagents: Protocatechuic (PRO), caffeic (CAF), p -coumaric (COU) and ferulic (FER) acids were purchased from Merck (Kenilworth, NJ, USA), chlorogenic (CHL) acid was obtained from Alfa Aesar (Haverhill, MA, USA), rosmarinic (ROS) acid, rutin (RUT) and quercetin (QUE) were acquired from Sigma Aldrich, luteolin (LUT) and kaempferol (KAE) were purchased from Roth (Dautphetal, Germany), while isoquercitrin (ISO) was obtained from HWI Analytik (Rülzheim, Germany).

Techniques: